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plasmids expressing cas9 pdd162  (Addgene inc)


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    Structured Review

    Addgene inc plasmids expressing cas9 pdd162
    Plasmids Expressing Cas9 Pdd162, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sgrna+expression/pDD162+(Peft-3%3A%3ACas9+%2B+Empty+sgRNA)+(Plasmid+%2347549)/pm41896213-178-26-31
    Average 95 stars, based on 260 article reviews
    plasmids expressing cas9 pdd162 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Expressing:

    Article Title: Genetic coupling of enhancer activity and connectivity in gene expression control
    Article Snippet: To generate cell lines expressing dCas9 KRAB, the lentiviral puromycin resistance vector pLV-dCas9-KRAB (Addgene plasmid #99372) was used. .. For sgRNA expression, we further used the lentiviral hygromycin resistance vector pLV-sgRNA (Addgene plasmid #62205). ..

    Article Title: Optimized small guide RNAs and methods of use
    Article Snippet: .. Exemplary suitable promoters for sgRNA expression are provided in e.g., Addgene, Cas9 expression cassettes can utilize a wide range of suitable promoters as known in the art. ..

    Article Title: Genetic coupling of enhancer activity and connectivity in gene expression control.
    Article Snippet: To generate cell lines expressing dCas9 KRAB, the lentiviral puromycin resistance vector pLV-dCas9-KRAB (Addgene plasmid #99372) was used. .. For sgRNA expression, we further used the lentiviral hygromycin resistance vector pLV-sgRNA (Addgene plasmid #62205). ..

    Article Title: A single microRNA miR-195 rescues the arrested B cell development induced by EBF1 deficiency
    Article Snippet: C57BL/6 mice were purchased from CLEA Japan Inc. NOD/Shi-scid,IL-2RγKO (NOG) and B6RG mice were purchased from Central Institute for Experimental Animals (CIEA). .. The Ebf1 −/+ mouse was originally generated by R. Grosschedl ( ). miR-195-deficient mice were generated based on the CRISPR/Cas9 system established by C. Gurumurthy , using pUC57-195sg-upstream and -downstream for sgRNA expression and pBGK (Addgene #65796) for Cas9 mRNA expression. ..

    Article Title: Phenazines are involved in the antagonism of a novel subspecies of Pseudomonas chlororaphis strain S1Bt23 against Pythium ultimum
    Article Snippet: .. S1Bt23 wild type, Phz+, Car r , Strept r , Kan s , tet s wild type, potent antagonist of Pythium ultimum This study S1Bt23DphzB mutant, DphzB, Phz- S1Bt23 derivative with phz B deleted This study S1Bt23DphzF mutant, DphzF, Phz- S1Bt23 derivative with phz F deleted This study Escherichia coli : E. coli DH5alpha Carrier of the plasmids Addgene Plasmids: pCasPA Tet r Expression of Cas9 nuclease and λ-Red system Addgene # 113347* pACRISPR car r , amp r , Kan s backbone for sgRNA expression kanamycin susceptible Addgene # 113348* pAKanCRISPR pACRISPR plus Kan r , amp s backbone for sgRNA expression with kanamycin resistance This study pGNW2 Kan r source of kanamycin resistant gene AddGene #122086* ..

    Article Title: The spatiotemporal distribution of LIN-5/NuMA regulates spindle orientation in the C. elegans germ line.
    Article Snippet: These fragments were assembled using NEBuilder to construct the donor plasmid, pTN26 (lin-5:: mAID::mNG). .. For Cas9 and sgRNA expression, a guide RNA sequence (50-GTCCAAGAAAAAGAACCGTC-30) targeting the C-terminal coding region of the lin-5 gene was used, as in Heppert et al.86 The plasmid, pTN27 (sg lin-5), was constructed by inverse PCR with the plasmid pDD162 (Dickinson et al.102, Addgene #47549). .. Editing of the lin-5 locus was performed as described previously.103 Briefly, 25 ng/mL of pTN26 and 5 ng/mL of pTN27were injected into the gonad of N2 animals with the control injectionmarkers pCFJ90 (Pmyo-2::mCherry, Addgene #8984, Frokjaer-Jensen et al.104) and pCFJ104 (Pmyo-3::mCherry, Addgene #19328, Frokjaer-Jensen et al.104).

    Article Title: Cooperative Architecture of Mitochondrial Proteome Homeostasis
    Article Snippet: Monoclonal HEK293T and U2OS cell lines stably expressing dCas9-KRAB-MeCP2 were established using the lenti_dCas9-KRAB-MeCP2 plasmid (Addgene 122205). .. For sgRNA expression, we used the pU6-sgRNA EF1Alpha-puro-T2A-BFP plasmid (Addgene 60955). ..

    Article Title: MOSAIC ATOH1 DELETION IN THE CHICK AUDITORY EPITHELIUM REVEALS A HOMEOSTATIC MECHANISM TO RESTORE HAIR CELL NUMBER.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Plasmid Preparation:

    Article Title: Genetic coupling of enhancer activity and connectivity in gene expression control
    Article Snippet: To generate cell lines expressing dCas9 KRAB, the lentiviral puromycin resistance vector pLV-dCas9-KRAB (Addgene plasmid #99372) was used. .. For sgRNA expression, we further used the lentiviral hygromycin resistance vector pLV-sgRNA (Addgene plasmid #62205). ..

    Article Title: Genetic coupling of enhancer activity and connectivity in gene expression control.
    Article Snippet: To generate cell lines expressing dCas9 KRAB, the lentiviral puromycin resistance vector pLV-dCas9-KRAB (Addgene plasmid #99372) was used. .. For sgRNA expression, we further used the lentiviral hygromycin resistance vector pLV-sgRNA (Addgene plasmid #62205). ..

    Article Title: The spatiotemporal distribution of LIN-5/NuMA regulates spindle orientation in the C. elegans germ line.
    Article Snippet: These fragments were assembled using NEBuilder to construct the donor plasmid, pTN26 (lin-5:: mAID::mNG). .. For Cas9 and sgRNA expression, a guide RNA sequence (50-GTCCAAGAAAAAGAACCGTC-30) targeting the C-terminal coding region of the lin-5 gene was used, as in Heppert et al.86 The plasmid, pTN27 (sg lin-5), was constructed by inverse PCR with the plasmid pDD162 (Dickinson et al.102, Addgene #47549). .. Editing of the lin-5 locus was performed as described previously.103 Briefly, 25 ng/mL of pTN26 and 5 ng/mL of pTN27were injected into the gonad of N2 animals with the control injectionmarkers pCFJ90 (Pmyo-2::mCherry, Addgene #8984, Frokjaer-Jensen et al.104) and pCFJ104 (Pmyo-3::mCherry, Addgene #19328, Frokjaer-Jensen et al.104).

    Article Title: Cooperative Architecture of Mitochondrial Proteome Homeostasis
    Article Snippet: Monoclonal HEK293T and U2OS cell lines stably expressing dCas9-KRAB-MeCP2 were established using the lenti_dCas9-KRAB-MeCP2 plasmid (Addgene 122205). .. For sgRNA expression, we used the pU6-sgRNA EF1Alpha-puro-T2A-BFP plasmid (Addgene 60955). ..

    Generated:

    Article Title: A single microRNA miR-195 rescues the arrested B cell development induced by EBF1 deficiency
    Article Snippet: C57BL/6 mice were purchased from CLEA Japan Inc. NOD/Shi-scid,IL-2RγKO (NOG) and B6RG mice were purchased from Central Institute for Experimental Animals (CIEA). .. The Ebf1 −/+ mouse was originally generated by R. Grosschedl ( ). miR-195-deficient mice were generated based on the CRISPR/Cas9 system established by C. Gurumurthy , using pUC57-195sg-upstream and -downstream for sgRNA expression and pBGK (Addgene #65796) for Cas9 mRNA expression. ..

    CRISPR:

    Article Title: A single microRNA miR-195 rescues the arrested B cell development induced by EBF1 deficiency
    Article Snippet: C57BL/6 mice were purchased from CLEA Japan Inc. NOD/Shi-scid,IL-2RγKO (NOG) and B6RG mice were purchased from Central Institute for Experimental Animals (CIEA). .. The Ebf1 −/+ mouse was originally generated by R. Grosschedl ( ). miR-195-deficient mice were generated based on the CRISPR/Cas9 system established by C. Gurumurthy , using pUC57-195sg-upstream and -downstream for sgRNA expression and pBGK (Addgene #65796) for Cas9 mRNA expression. ..

    Mutagenesis:

    Article Title: Phenazines are involved in the antagonism of a novel subspecies of Pseudomonas chlororaphis strain S1Bt23 against Pythium ultimum
    Article Snippet: .. S1Bt23 wild type, Phz+, Car r , Strept r , Kan s , tet s wild type, potent antagonist of Pythium ultimum This study S1Bt23DphzB mutant, DphzB, Phz- S1Bt23 derivative with phz B deleted This study S1Bt23DphzF mutant, DphzF, Phz- S1Bt23 derivative with phz F deleted This study Escherichia coli : E. coli DH5alpha Carrier of the plasmids Addgene Plasmids: pCasPA Tet r Expression of Cas9 nuclease and λ-Red system Addgene # 113347* pACRISPR car r , amp r , Kan s backbone for sgRNA expression kanamycin susceptible Addgene # 113348* pAKanCRISPR pACRISPR plus Kan r , amp s backbone for sgRNA expression with kanamycin resistance This study pGNW2 Kan r source of kanamycin resistant gene AddGene #122086* ..

    Sequencing:

    Article Title: The spatiotemporal distribution of LIN-5/NuMA regulates spindle orientation in the C. elegans germ line.
    Article Snippet: These fragments were assembled using NEBuilder to construct the donor plasmid, pTN26 (lin-5:: mAID::mNG). .. For Cas9 and sgRNA expression, a guide RNA sequence (50-GTCCAAGAAAAAGAACCGTC-30) targeting the C-terminal coding region of the lin-5 gene was used, as in Heppert et al.86 The plasmid, pTN27 (sg lin-5), was constructed by inverse PCR with the plasmid pDD162 (Dickinson et al.102, Addgene #47549). .. Editing of the lin-5 locus was performed as described previously.103 Briefly, 25 ng/mL of pTN26 and 5 ng/mL of pTN27were injected into the gonad of N2 animals with the control injectionmarkers pCFJ90 (Pmyo-2::mCherry, Addgene #8984, Frokjaer-Jensen et al.104) and pCFJ104 (Pmyo-3::mCherry, Addgene #19328, Frokjaer-Jensen et al.104).

    Article Title: MOSAIC ATOH1 DELETION IN THE CHICK AUDITORY EPITHELIUM REVEALS A HOMEOSTATIC MECHANISM TO RESTORE HAIR CELL NUMBER.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Construct:

    Article Title: The spatiotemporal distribution of LIN-5/NuMA regulates spindle orientation in the C. elegans germ line.
    Article Snippet: These fragments were assembled using NEBuilder to construct the donor plasmid, pTN26 (lin-5:: mAID::mNG). .. For Cas9 and sgRNA expression, a guide RNA sequence (50-GTCCAAGAAAAAGAACCGTC-30) targeting the C-terminal coding region of the lin-5 gene was used, as in Heppert et al.86 The plasmid, pTN27 (sg lin-5), was constructed by inverse PCR with the plasmid pDD162 (Dickinson et al.102, Addgene #47549). .. Editing of the lin-5 locus was performed as described previously.103 Briefly, 25 ng/mL of pTN26 and 5 ng/mL of pTN27were injected into the gonad of N2 animals with the control injectionmarkers pCFJ90 (Pmyo-2::mCherry, Addgene #8984, Frokjaer-Jensen et al.104) and pCFJ104 (Pmyo-3::mCherry, Addgene #19328, Frokjaer-Jensen et al.104).

    Inverse PCR:

    Article Title: The spatiotemporal distribution of LIN-5/NuMA regulates spindle orientation in the C. elegans germ line.
    Article Snippet: These fragments were assembled using NEBuilder to construct the donor plasmid, pTN26 (lin-5:: mAID::mNG). .. For Cas9 and sgRNA expression, a guide RNA sequence (50-GTCCAAGAAAAAGAACCGTC-30) targeting the C-terminal coding region of the lin-5 gene was used, as in Heppert et al.86 The plasmid, pTN27 (sg lin-5), was constructed by inverse PCR with the plasmid pDD162 (Dickinson et al.102, Addgene #47549). .. Editing of the lin-5 locus was performed as described previously.103 Briefly, 25 ng/mL of pTN26 and 5 ng/mL of pTN27were injected into the gonad of N2 animals with the control injectionmarkers pCFJ90 (Pmyo-2::mCherry, Addgene #8984, Frokjaer-Jensen et al.104) and pCFJ104 (Pmyo-3::mCherry, Addgene #19328, Frokjaer-Jensen et al.104).

    Modification:

    Article Title: MOSAIC ATOH1 DELETION IN THE CHICK AUDITORY EPITHELIUM REVEALS A HOMEOSTATIC MECHANISM TO RESTORE HAIR CELL NUMBER.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Cloning:

    Article Title: MOSAIC ATOH1 DELETION IN THE CHICK AUDITORY EPITHELIUM REVEALS A HOMEOSTATIC MECHANISM TO RESTORE HAIR CELL NUMBER.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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